Licht-im-Terrarium: Literaturdatenbank |
Loew, R., Govardovskii, V. I., Röhlich, P., & Szél, Á. (1996). Vitamin d in foods and as supplements. Progress in Biophysics and Molecular Biology, 13(2), 247–256. Added by: Sarina (2010-01-08 21:48:24) Last edited by: Sarina (2010-01-08 22:19:48) |
Resource type: Journal Article DOI: 10.1017/S0952523800007483 BibTeX citation key: Loew1996 View all bibliographic details |
Categories: Englisch = English Keywords: Echsen = Lizards, Farbsehen = Color Vision, Netzhaut = Retina, Ultraviolett = Ultraviolet Creators: Govardovskii, Loew, Röhlich, Szél Collection: Progress in Biophysics and Molecular Biology |
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Abstract |
Retinas of the nocturnal geckos, Hemidactylus turcicus, Hemidactylus garnotii, and Teratoscincus scincus, were studied with microspectrophotometry and immunocytochemistry against various visual pigment epitopes to reveal UV-sensitive photoreceptors. From 6-20% of the thinner members of type C double photoreceptors, earlier believed to be blue-sensitive, were found to contain a UV-absorbing visual pigment with lambda max at 363-366 nm. The pigment had bleaching and dichroic properties typical of other photoreceptor cell types of the retina. Presumptive UV-sensitive cells in retinal sections were "negatively" labeled as they did not react with either the cone-specific monoclonal antibody COS-1 or with the anti-rhodopsin polyclonal serum AO, which together labeled all of the remaining photoreceptor types (green-sensitive A singles, B doubles, and thicker members of C doubles, as well as the blue-sensitive majority of thinner members of C doubles). UV cells were moderately stained with the mAb K42-41 produced against the 5-6 loop of bovine rhodopsin, which also moderately labeled blue-sensitive cells. mAb OS-2 strongly stained all outer segments, including the UV-sensitive ones. Similarities between gecko UV visual pigments, and UV visual pigments of other vertebrates, as well as possible functional significance of these cells are discussed.
Added by: Sarina Last edited by: Sarina |